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KMID : 0624620090420020081
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BMB Reports
2009 Volume.42 No. 2 p.81 ~ p.85
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Microplate hybridization assay for detection of isoniazid resistance in Mycobacterium tuberculosis
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Han Hye-Eun
Hwang Joo-Hwan
Bang Hye-Eun
Kim Yeun
Cho Sang-Nae
Kim Tae-Ue
Lee Hye-Young
Lee In-Soo
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Abstract
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Early and accurate detection of drug resistant Mycobacterium tuberculosis can improve both the treatment outcome and public health control of tuberculosis. A number of molecular-based techniques have been developed including ones using probe molecules that target drug resistance-related mutations. Although these techniques are highly specific and sensitive, mixed signals can be obtained when the drug resistant isolates are mixed with drug susceptible isolates. In order to overcome this problem, we developed a new drug susceptibility test (DST) for one of the most effective anti-tuberculosis drug, isoniazid. This technique employed a microplate hybridization assay that quantified signals from each probe molecule, and was evaluated using clinical isolates. The evaluation analysis clearly showed that the microplate hybridization assay was an accurate and rapid method that overcame the limitations of DST based on conventional molecular techniques.
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KEYWORD
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Isoniazid (INH), MDR-TB, Microplate hybridization assay
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